myGenomeBrowser
Toggle navigation
myGenomeBrowser
Xcf7767Rpb
       
Browse
       
Open in a new window
       
Search
       
Blast
       
Extract
Choose a target database
Xcf7767Rpb genomic sequences - Nucleotide,3 refseq sequences
prokka cds - Nucleotide,4511 cds sequences
prokka gene - Nucleotide,4579 gene sequences
prokka mrna - Nucleotide,4511 mrna sequences
prokka ncrna - Nucleotide,68 ncrna sequences
prokka prot - Protein,4511 prot sequences
choose a program
blastn: Nucleotide-Nucleotide BLAST
blastp: Protein-Protein BLAST
blastx: Translated Query-Protein Subject BLAST
tblastn: Protein Query-Translated Subject BLAST
tblastx: Translated Query-Translated Subject BLAST
blastp options
Input query options
Location on the query sequence in 1-based offsets (Format: start-stop)
General search options
Expectation value (E) threshold for saving hits
Cost to open a gap
Cost to extend a gap
Task to execute
blastp
blastp-short
deltablast
Word size for wordfinder algorithm (length of best perfect match)
Scoring matrix name (normally BLOSUM62)
BLOSUM62
PAM70
BLOSUM80
PAM30
BLOSUM45
Minimum word score such that the word is added to the BLAST lookup table
Use composition-based statistics
no composition-based statistics
Composition-based score adjustment as in Bioinformatics 21:902-911, 2005, unconditionally
Composition-based score adjustment as in Bioinformatics 21:902-911, 2005, conditioned on sequence properties
Composition-based statistics as in NAR 29:2994-3005, 2001
Formatting options
Show NCBI GIs in deflines
Number of database sequences to show alignments for
Query filtering options
Apply filtering locations as soft masks
Use lower case filtering in query and subject sequence(s)
Filter query sequence with SEG (Format: 'yes', 'window locut hicut', or 'no' to disable)
Restrict search or results
Filtering algorithm ID to apply to the BLAST database as soft masking
Filtering algorithm ID to apply to the BLAST database as hard masking
Percent identity
If the query range of a hit is enveloped by that of at least this many higher-scoring hits, delete the hit
Best Hit algorithm overhang value(recommended value : 0.1)
Best Hit algorithm score edge value(recommended value : 0.1)
Maximum number of aligned sequences to keep
Statistical options
Effective length of the database
Effective length of the search space
Override maximum number of HSPs per subject to save for ungapped searches (0 means do not override)
Extension options
X-dropoff value (in bits) for ungapped extensions
Multiple hits window size, use 0 to specify 1-hit algorithm
X-dropoff value (in bits) for preliminary gapped extensions
X-dropoff value (in bits) for final gapped alignment
Perform ungapped alignment only?
Miscellaneous options
Compute locally optimal Smith-Waterman alignments?
blastn options
Input query options
Location on the query sequence in 1-based offsets (Format: start-stop)
Query strand(s) to search against database/subject
both
minus
plus
General search options
Expectation value (E) threshold for saving hits
Cost to open a gap
Cost to extend a gap
Task to execute
dc-megablast
rmblastn
blastn-short
blastn
megablast
Word size for wordfinder algorithm (length of best perfect match)
Penalty for a nucleotide mismatch
Reward for a nucleotide match
Formatting options
Show NCBI GIs in deflines
Number of database sequences to show alignments for
Query filtering options
Apply filtering locations as soft masks
Use lower case filtering in query and subject sequence(s)
Filter query sequence with DUST(Format :'yes','level window linker', or 'no' to disable)
BLAST database containing filtering elements(i . e . : repeats)
Enable WindowMasker filtering using a Taxonomic ID
Enable WindowMasker filtering using this repeats database .
Restrict search or results
Filtering algorithm ID to apply to the BLAST database as soft masking
Filtering algorithm ID to apply to the BLAST database as hard masking
Percent identity
If the query range of a hit is enveloped by that of at least this many higher-scoring hits, delete the hit
Best Hit algorithm overhang value(recommended value : 0.1)
Best Hit algorithm score edge value(recommended value : 0.1)
Maximum number of aligned sequences to keep
Statistical options
Effective length of the database
Effective length of the search space
Override maximum number of HSPs per subject to save for ungapped searches (0 means do not override)
Extension options
X-dropoff value (in bits) for ungapped extensions
Multiple hits window size, use 0 to specify 1-hit algorithm
X-dropoff value (in bits) for preliminary gapped extensions
X-dropoff value (in bits) for final gapped alignment
Use non-greedy dynamic programming extension
Minimum raw gapped score to keep an alignment in the preliminary gapped and traceback stages
Perform ungapped alignment only?
Number of off-diagonals to search for the 2nd hit, use 0 to turn off
Discontiguous MegaBLAST options
Discontiguous MegaBLAST template type
coding
coding_and_optimal
optimal
Discontiguous MegaBLAST template length
18
21
16
blastx options
Input query options
Location on the query sequence in 1-based offsets (Format: start-stop)
Query strand(s) to search against database/subject
both
minus
plus
Genetic code to use to translate query
Vertebrate Mitochondrial (2)
Standard (1)
Flatworm Mitochondrial (14)
Echinoderm Mitochondrial (9)
Euplotid Nuclear (10)
Invertebrate Mitochondrial (5)
Bacteria and Archaea (11)
Alternative Yeast Nuclear (12)
Yeast Mitochondrial (3)
Mold Mitochondrial; ... (4)
Blepharisma Macronuclear (15)
Ciliate Nuclear; ... (6)
Ascidian Mitochondrial (13)
General search options
Expectation value (E) threshold for saving hits
Cost to open a gap
Cost to extend a gap
Word size for wordfinder algorithm (length of best perfect match)
Length of the largest intron allowed in a translated nucleotide sequence when linking multiple distinct alignments (a negative value disables linking)
Scoring matrix name (normally BLOSUM62)
PAM30
BLOSUM45
BLOSUM80
PAM70
BLOSUM62
Minimum word score such that the word is added to the BLAST lookup table
Use composition-based statistics
no composition-based statistics
Composition-based score adjustment as in Bioinformatics 21:902-911, 2005, unconditionally
Composition-based score adjustment as in Bioinformatics 21:902-911, 2005, conditioned on sequence properties
Composition-based statistics as in NAR 29:2994-3005, 2001
Formatting options
Show NCBI GIs in deflines
Number of database sequences to show alignments for
Query filtering options
Apply filtering locations as soft masks
Use lower case filtering in query and subject sequence(s)
Filter query sequence with SEG (Format: 'yes', 'window locut hicut', or 'no' to disable)
Restrict search or results
Filtering algorithm ID to apply to the BLAST database as soft masking
Filtering algorithm ID to apply to the BLAST database as hard masking
Percent identity
If the query range of a hit is enveloped by that of at least this many higher-scoring hits, delete the hit
Best Hit algorithm overhang value(recommended value : 0.1)
Best Hit algorithm score edge value(recommended value : 0.1)
Maximum number of aligned sequences to keep
Statistical options
Effective length of the database
Effective length of the search space
Override maximum number of HSPs per subject to save for ungapped searches (0 means do not override)
Extension options
X-dropoff value (in bits) for ungapped extensions
Multiple hits window size, use 0 to specify 1-hit algorithm
X-dropoff value (in bits) for preliminary gapped extensions
X-dropoff value (in bits) for final gapped alignment
Perform ungapped alignment only?
Miscellaneous options
Compute locally optimal Smith-Waterman alignments?
tblastn options
Input query options
Location on the query sequence in 1-based offsets (Format: start-stop)
General search options
Expectation value (E) threshold for saving hits
Cost to open a gap
Cost to extend a gap
Word size for wordfinder algorithm (length of best perfect match)
Genetic code to use to translate database/subjects
Blepharisma Macronuclear (15)
Ascidian Mitochondrial (13)
Ciliate Nuclear; ... (6)
Euplotid Nuclear (10)
Invertebrate Mitochondrial (5)
Bacteria and Archaea (11)
Yeast Mitochondrial (3)
Alternative Yeast Nuclear (12)
Mold Mitochondrial; ... (4)
Echinoderm Mitochondrial (9)
Vertebrate Mitochondrial (2)
Flatworm Mitochondrial (14)
Standard (1)
Length of the largest intron allowed in a translated nucleotide sequence when linking multiple distinct alignments (a negative value disables linking)
Scoring matrix name (normally BLOSUM62)
BLOSUM45
PAM30
PAM70
BLOSUM80
BLOSUM62
Minimum word score such that the word is added to the BLAST lookup table
Formatting options
Show NCBI GIs in deflines
Number of database sequences to show alignments for
Query filtering options
Apply filtering locations as soft masks
Use lower case filtering in query and subject sequence(s)
Filter query sequence with SEG (Format: 'yes', 'window locut hicut', or 'no' to disable)
Restrict search or results
Filtering algorithm ID to apply to the BLAST database as soft masking
Filtering algorithm ID to apply to the BLAST database as hard masking
Percent identity
If the query range of a hit is enveloped by that of at least this many higher-scoring hits, delete the hit
Best Hit algorithm overhang value(recommended value : 0.1)
Best Hit algorithm score edge value(recommended value : 0.1)
Maximum number of aligned sequences to keep
Statistical options
Effective length of the database
Effective length of the search space
Override maximum number of HSPs per subject to save for ungapped searches (0 means do not override)
Extension options
X-dropoff value (in bits) for ungapped extensions
Multiple hits window size, use 0 to specify 1-hit algorithm
tblastx options
Input query options
Location on the query sequence in 1-based offsets (Format: start-stop)
Query strand(s) to search against database/subject
minus
plus
both
Genetic code to use to translate query
Echinoderm Mitochondrial (9)
Standard (1)
Flatworm Mitochondrial (14)
Vertebrate Mitochondrial (2)
Ciliate Nuclear; ... (6)
Blepharisma Macronuclear (15)
Ascidian Mitochondrial (13)
Yeast Mitochondrial (3)
Alternative Yeast Nuclear (12)
Mold Mitochondrial; ... (4)
Bacteria and Archaea (11)
Invertebrate Mitochondrial (5)
Euplotid Nuclear (10)
General search options
Expectation value (E) threshold for saving hits
Cost to open a gap
Cost to extend a gap
Word size for wordfinder algorithm (length of best perfect match)
Scoring matrix name (normally BLOSUM62)
PAM70
BLOSUM80
BLOSUM62
PAM30
BLOSUM45
Length of the largest intron allowed in a translated nucleotide sequence when linking multiple distinct alignments (a negative value disables linking)
Minimum word score such that the word is added to the BLAST lookup table
Genetic code to use to translate database/subjects
Blepharisma Macronuclear (15)
Ciliate Nuclear; ... (6)
Ascidian Mitochondrial (13)
Bacteria and Archaea (11)
Invertebrate Mitochondrial (5)
Alternative Yeast Nuclear (12)
Yeast Mitochondrial (3)
Mold Mitochondrial; ... (4)
Euplotid Nuclear (10)
Echinoderm Mitochondrial (9)
Vertebrate Mitochondrial (2)
Flatworm Mitochondrial (14)
Standard (1)
Formatting options
Show NCBI GIs in deflines
Number of database sequences to show alignments for
Query filtering options
Apply filtering locations as soft masks
Use lower case filtering in query and subject sequence(s)
Filter query sequence with SEG (Format: 'yes', 'window locut hicut', or 'no' to disable)
Restrict search or results
Filtering algorithm ID to apply to the BLAST database as soft masking
Filtering algorithm ID to apply to the BLAST database as hard masking
Percent identity
If the query range of a hit is enveloped by that of at least this many higher-scoring hits, delete the hit
Best Hit algorithm overhang value(recommended value : 0.1)
Best Hit algorithm score edge value(recommended value : 0.1)
Maximum number of aligned sequences to keep
Statistical options
Effective length of the database
Effective length of the search space
Override maximum number of HSPs per subject to save for ungapped searches (0 means do not override)
Extension options
X-dropoff value (in bits) for ungapped extensions
Multiple hits window size, use 0 to specify 1-hit algorithm